Practice Quiz

HT Histotechnician

Category - Fixation

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Which of the following best describes fixation?
  1. A procedure adopted to kill, harden and preserve the tissue for microscopic study by the use of substances like xylene.
  2. A procedure adopted to kill, harden and preserve the tissue for microscopic study by the use of substances like mercuric chloride.
  3. The procedure in which the ionic materials are removed from the tissue.
  4. The process of removing intracellular and extracellular H2O.
Explanation
Answer: B - A procedure adopted to kill, harden and preserve the tissue for microscopic study by the use of substances like mercuric chloride.
Fixation is best described as a procedure adopted to kill, harden and preserve the tissue for microscopic study by the use of substances like aldehydes (formaldehyde and glutaraldehyde), metallic fixatives (mercuric chloride), lead fixatives and other compound fixatives. Xylene is not used as fixative but rather as a clearing agent for de-alcoholization in the embedding process. Letter C is the description of the procedure that removes calcium from the tissue called as decalcification and letter D is the description of the process of removal of water in the tissue called as dehydration.
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