HT Histotechnician

Category - Fixation

Which of the following is not an example of a nuclear fixative?
  1. Flemming’s fluid
  2. Newcomer’s fluid
  3. Heidenhain’s Susa
  4. Orth’s fluid
Explanation
Answer: D - Orth’s fluid
The examples of nuclear fixatives are Felmming’s fluid, Newcomer’s Fluid, Heidenhain’s Susa, Carnoy’s fluid and Bouin’s fluid. These nuclear fixatives contain glacial acetic acid as their primary component due to its affinity for nuclear chromatin. They have a pH of 4.6 or less. Orth’s fluid is an example of a cytoplasmic fixative that never contains the glacial acetic acid which destroys mitochondria and golgi bodies of the cytoplasm.
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