Practice Quiz

HT Histotechnician

Category - Fixation

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What is the usual fixation time when using 10% formaldehyde?
  1. 3 hours
  2. 12 hours
  3. 24 hours
  4. 72 hours
Explanation
Answer: C - 24 hours
The usual fixation time when using 10% formaldehyde is 24 hours. This is because it produces excellent morphological detail. “Over fixing” the tissue with formaldehyde could complicate sectioning and impair the staining process. On the other hand, “under fixing” with formaldehyde can produce some artifacts because of its exposure to alcohol. Other durations in the choices are not good fixation times when using formaldehyde.
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