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HT Histotechnician

Category - Staining

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What is the most useful metachromatic dye for acid mucin?

Azure A
Azure B
Toluidine blue
Alcian blue

Explanation

Answer: A - Azure A

The most useful metachromatic dye for acid mucin is Azure A. In Azure A metachromatic staining, the intensity of staining appears to be due to the initial potassium permanganate oxidation step which helps the tissue to take up the dye. The other choices are not useful metachromatic dyes used for acid mucin.

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