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HT Histotechnician

Category - Staining

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What fat stain was popularized by Lilie and Ashburn in 1943?

Sudan III
Sudan Black B
Sudan IV
Oil Red O

Explanation

Answer: D - Oil Red O
The fat stain that was popularized by Lilie and Ashburn in 1943 is Oil Red O. It is used as a 50% and 60% fresh aqueous dilution of a saturated 99% isopropanol stock solution. Oil Red O is use to stain neutral fats and lipofuchsin. The other fat stains were not popularized by Lilie and Ashburn in 1943.

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