HT Histotechnician

Category - Fixation

All of the following choices below are fixatives of choice for electron microscopy, except:
  1. 100% Methanol
  2. Osmium Tetroxide
  3. Glutaraldehyde
  4. Paraformaldehyde
Explanation
Answer: A - 100% Methanol
All of the following choices above are fixatives of choice for electron microscopy except for 100% Methanol. Methanol penetrates the tissue gradually and might not penetrate the tissue deeper. It is only recommended for the preservation of dry and wet smears.
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