HT Histotechnician

Category - Staining

In the combined Alcian Blue-PAS Technique for acid and neutral mucins, this alum hematoxylin solution should not be use as a counterstain. What is this alum hematoxylin solution?
  1. Ehrlich’s hematoxylin
  2. Harris hematoxylin
  3. Cole’s hematoxylin
  4. Mayer’s hematoxylin
Explanation
Answer: A - Ehrlich’s hematoxylin

In the combined Alcian Blue-PAS Technique for acid and neutral mucins, Ehrlich’s hematoxylin should not be use as a counterstain. This solution should be avoided for it will also stain mucin and will complicate with the final result. The other choices do not fit the description stated in the question.
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