Answer: D - Krajan’s
All of the following choices are Romanowsky stains except for Krajan’s. This is a rapid method for staining elastic fibers.
Romanowsky stains are a group of histological stains used to visualize blood cells and other biological specimens under a microscope. These stains were developed by Polish physician Paul Ehrlich and Russian physician Dmitri Romanowsky in the late 19th and early 20th centuries.
Romanowsky stains are commonly used to stain blood films for the diagnosis of blood-related disorders, such as anemia, leukemia, and malaria. They are named after Romanowsky because he modified Ehrlich's original formulation to improve the staining properties and make the stain more selective.
There are two types of Romanowsky stains: Wright-Giemsa and Jenner-Giemsa. Wright-Giemsa stain is the most commonly used Romanowsky stain and is made up of a mixture of eosin and methylene blue dyes. Jenner-Giemsa stain is a modification of Wright-Giemsa stain that uses azure B dye instead of methylene blue.
Leishman is a type of Romanowsky stain. It was developed by Scottish pathologist William Boog Leishman in 1901 as a modification of Romanowsky's stain. The Leishman stain is a mixture of eosin and methylene blue dyes, similar to Wright-Giemsa stain, which is another commonly used Romanowsky stain.
Like other Romanowsky stains, the Leishman stain is used to visualize blood cells and other biological specimens under a microscope. It is commonly used to stain blood films and bone marrow aspirates for the diagnosis of blood-related disorders such as anemia, leukemia, and malaria. The specific staining pattern of the Leishman stain can provide valuable diagnostic information for these disorders.
In addition to its use in the diagnosis of blood-related disorders, the Leishman stain is also used in other fields such as microbiology and parasitology to stain various types of specimens for microscopic examination.
Romanowsky stains work by staining different components of blood cells in different colors, allowing for easy identification and classification of the different types of blood cells. Red blood cells appear pink or orange, white blood cells appear blue, and platelets appear purple. The specific staining pattern of Romanowsky stains can provide valuable diagnostic information for blood-related disorders.
