HT Histotechnician

Category - Staining

What is the use of a mordant?
  1. To form a bridge between the stain used and the tissue to be stained.
  2. To decolorize the stained tissue.
  3. To act as a counterstain that will stain the tissue with pinkish to red and the background as blue.
  4. To combine aqueous solutions of acid and basic dyes.
Explanation
Answer: A - To form a bridge between the stain used and the tissue to be stained.

The use of a mordant is to form a bridge between the stain used and the tissue to be stained. This will allow the staining reaction to occur. Without the use of Mordants, no staining could possibly take place. All the other options do not define the function of a mordant.
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