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HT Histotechnician

Category - Staining

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It is an oxazine dye used as an alternative to iron hematoxylin nuclear stain?

Celestine blue
Trypan blue
Nile blue
Toluidine blue

Explanation

Answer: A - Celestine blue
An oxazine dye used as an alternative to iron hematoxylin nuclear stain is Celestine blue. It produces a strong and precise nuclear stain that is resistant to decolorization by succeeding acid stains and solution. Celestine blue forms a strong staining lake with iron alum, acting as a mordant to bind hematoxylin. All the other choices are not used as an alternative to iron hematoxylin nuclear stain.

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