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HT Histotechnician

Category - Staining

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What is the only substance that can fix, differentiate or stain tissues all by itself?

Trichloroacetic acid
Picric acid
Methylene blue
Saffron

Explanation

Answer: B - Picric acid

The only substance that can fix, differentiate or stain tissues all by itself is Picric Acid. It may be use as a counterstain to basic cytoplasmic stains, to acid fuschin in staining of connective tissue by Van Gieson’s or to crystal violet in the microscopic study of fungi. It can be use also as a fixative, as a decalcifying agent and as a tissue softener.

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