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HT Histotechnician

Category - Staining

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This fluorescent stain is used to demonstrate acid mucins. What do you call this stain?

Methylene blue
Acridine orange
Alcian blue
Rhodamine B

Explanation

Answer: B - Acridine orange
The fluorescent stain that is used to demonstrate acid mucins is called Acridine orange. When stained with acridine orange, a section stained with iron hematoxylin will give a brilliant orange fluorescence. The other stains in the choices above are not used to demonstrate acid mucins.

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