HT Histotechnician

Category - Staining

Harris' solution is an alum hematoxylin. Which of the following is used to oxidize Harris' solution?

  1. Mercuric chloride
  2. Sodium iodate
  3. Ferric chloride
  4. Potassium iodate
Explanation

Answers: B and D - Sodium iodate or potassium iodate are used to oxidize Harris' solution

For Harris' hematoxylin solution, the oxidizing agent can be either sodium or potassium iodate. Mercuric chloride was previously used to oxidize Harris' solution, but is no longer used due to its toxicity. Ferric chloride is used to oxidize Weigert's solution, an iron hematoxylin.

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