HT Histotechnician

Category - Staining

The most commonly used fluorochrome to demonstrate DNA and RNA in fresh or fixed tissues is?
  1. Janus green B
  2. Acridine orange
  3. Victoria blue
  4. Night blue
Explanation
Answer: B - Acridine orange
The most commonly used fluorochrome to demonstrate DNA and RNA in fresh or fixed tissues is Acridine orange. RNA is stained brick to orange red while DNA emits a yellow-green fluorescence. Other choices are not commonly used to demonstrate RNA and DNA in tissue samples.
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