HT Histotechnician

Category - Staining

In high pH Congo red technique for amyloid, the solutions used are buffered to what pH?
  1. 7
  2. 8
  3. 9
  4. 10
Explanation
Answer: D - 10
In high pH Congo red technique for amyloid, the solutions used are buffered to an alkaline pH of 10. The technique uses alkaline alcoholic solvents combined with high concentration of salts to produce a Congo red solution and is buffered to ph of 10. Other pH values are incorrect and are not used in this technique.
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