Practice Quiz

HT Histotechnician

Category - Staining

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In the Feulgen technique of acid hydrolysis, how many percent of alcoholic solution of Acriflavine is needed in order to be used as an alternative to basic fuchsin in Schiff’s reagent?
  1. 0.01 %
  2. 0.02 %
  3. 0.03 %
  4. 0.04 %
Explanation
Answer: A - 0.01 %
In the Feulgen technique of acid hydrolysis, the percentage of alcoholic solution of Acriflavine needed in order to be used as an alternative to basic fuchsin in Schiff’s reagent is 0.01 %. In this technique DNA is stained a fluorescent yellow color. All the other percentages are inaccurate.
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