HT Histotechnician

Category - Microtomy

In cutting the tissue block, a hole is observed in the section. What do you think is the reason for this?
  1. Sections are too thin
  2. Incomplete dehydration, clearing or infiltration of tissue with wax
  3. Sections are too thick
  4. Dirt was formed in the embedding medium
Explanation
Answer: D - Dirt was formed in the embedding medium
In cutting the tissue block, a hole is observed in the section. The reason for it is that dirt was formed in the embedding tissue so there was a space or section that was not covered by paraffin. Other reasons stated in the choices above do not accurately explain the reason why a hole is observed in the section.
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