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HT Histotechnician

Category - Processing and Embedding

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Which of the following acids is a moderate-acting decalcifying agent with less distortion of the sample tissue?

Citric acid
Formic acid
Hydrochloric acid
Trichloroacetic acid

Explanation

Answer: B - Formic acid
Formic acid is the acid that is a moderate-acting decalcifying agent with less distortion of the sample tissue. Formic acid is the second fastest decalcifying agent that produces better nuclear staining with less distortion of the tissue compared to nitric acid. It is also recommended for routine decalcification of postmortem research tissue. All the other choices are not moderate acting acids and they do not lessen tissue distortion.

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