HT Histotechnician

Category - Staining

What stain is used to demonstrate deposits of calcium salts and possible sites of phosphatase activities?
  1. Acridine orange
  2. Acridine Red 3B
  3. Alcian blue
  4. Aniline blue
Explanation
Answer: B - Acridine Red 3B

The stain used to demonstrate deposits of calcium salts and possible sites of phosphatase activities is Acridine Red 3B. The other choices are used for the differential staining of the tissues. Acridine orange is used to stain RNA and DNA, Alcian blue is used to stain connective tissues, and lastly Aniline blue is used for counterstaining of epithelial sections.
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