HT Histotechnician

Category - Microtomy

What is the usual range of thickness in a cut of tissue for routine histologic procedures?
  1. 0.5 - 1.0 microns
  2. 1.0 - 2.0 microns
  3. 3.0 - 4.5 microns
  4. 4.0 - 6.0 microns
Explanation
Answer: D - 4.0 - 6.0 microns
The usual range of thickness in a cut of tissue for routine histologic procedures is 4.0 - 6.0 microns. The other ranges do not reflect the ideal thickness of the cut of tissue. The micrometer gauge is set to the required thickness and the knife is positioned in such a way that the center of the blade is in line with the block and the knife has been securely clamped in place. The other options do not reflect the ideal thickness range of a cut of tissue.
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