HT Histotechnician

Category - Staining

What iron hematoxylin solution uses ferric ammonium sulfate as a mordant?
  1. Weigert’s
  2. Heidenhain’s
  3. PTAH
  4. All of the above
Explanation
Answer: B - Heidenhain’s

The iron hematoxylin solution that uses ferric ammonium sulfate as a mordant is Heidenhain’s. The mordant differentiator is not added to the solution and is used separately. The dye-lake will color intense blue-black. All the other choices do not use ferric ammonium sulfate as a mordant.
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