HT Histotechnician

Category - Staining

What does ripening refer to in the context of Hematoxylin staining?
  1. Combination of Hematoxylin to other stains
  2. Oxidation of Hematoxylin
  3. Use of Hematoxylin stain as a regressive stain
  4. To form a bridge between other stains
Explanation
Answer: B - Oxidation of Hematoxylin

Ripening refers to the process whereby Hematoxylin undergoes the process of oxidation. This is usually done by exposing the substance to air and sunlight. All the other choices do not accurately describe what ripening is, in the context of hematoxylin staining.
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