HT Histotechnician

Category - Staining

This stain solution is commercially prepared. This is prepared with the mixture of saturated solutions of methylene blue in alcohol, potassium hydroxide and distilled water. What is this staining solution?
  1. Loffler’s Polychrome Methylene blue
  2. Lugol’s iodine
  3. Malachite green
  4. Best Carmine working solution
Explanation
Answer: A - Loffler’s Polychrome Methylene Blue
The stain solution that is commercially prepared is Loffler’s Polychrome Methylene blue. This is prepared with the mixture of saturated solutions of methylene blue in alcohol, potassium hydroxide and distilled water. This stain is used for rapid diagnosis and for frozen sections. The other choices do not fit the description in the question.
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