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HT Histotechnician

Category - Staining

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This is a histochemical method that is based on the unmasking of ferric ion by using diluted hydrochloric acid, which is a component of acid ferrocyanide solution?

Perl’s Prussian Blue
Gomori’s Prussian Blue
Turnbull’s Blue Reaction
Benzidine-Nitroprusside stain

Explanation

Answer: A - Perl’s Prussian blue

The histochemical method that is based on the unmasking of ferric ion by using diluted hydrochloric acid, which is a component of acid frrocyanide solution, is Perl’s Prussian blue. The ferric ion then reacts with dilute potassium ferrocyanide solution to produce an insoluble blue compound ferric ferrocyanide or Prussian blue. The other choices do not fit the description stated in the question.

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