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HT Histotechnician

Category - Staining

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The components of this dye are capable of differentiating two lipid classes. What is this dye?

Methylene blue
Nile blue sulfate
Osmic acid
Aniline blue

Explanation

Answer: B - Nile blue sulfate
Nile blue sulfate is the dye that is capable of differentiating two lipid classes. The two components are red oxazone, which dissolves neutral lipids and blue oxazine, which reacts with phospholipids and free fatty acids.

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