HT Histotechnician

Category - Staining

It is an acid dye that stains basic protamines and histones?
  1. Fast green
  2. Nile blue
  3. Prussian blue
  4. Methyl green
Explanation
Answer: A - Fast green
The acid dye that stains basic protamines and histones is Fast green. These basic groups of tissue have higher isoelectrical points than the pH of the staining solutions which enable them to stain with Fast green. All other proteins that have lower isoelectrical points will not be stained. The other choices are not used to stain basic protamines and histones.
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