HT Histotechnician

Category - Staining

In what method of staining does the dye taken up by the tissue do not become washed or decolorized?
  1. Regressive staining
  2. Direct staining
  3. Progressive staining
  4. Metachromatic staining
Explanation
Answer: C - Progressive staining
The method of staining wherein the dye taken up by the tissue do not become washed or decolorized is Progressive staining. In this process, the tissue elements are stained in a definite sequence and the staining solution is applied for specific periods of time. Once the dye is taken up by the tissue, it is not washed or decolorized. The differentiation of tissue detail relies solely on the selective affinity of the dye for different cellular elements. Other methods of staining in the choices do not fit the description in the question.
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