Practice Quiz

HT Histotechnician

Category - Microtomy

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In the cold knife procedure, there is a term called as “Dew Line”. What do you think is the meaning of this?
  1. Different temperatures between the tissue and the knife
  2. The point at which sections may then be cut at 10 microns of thickness
  3. Optimum condition for sectioning
  4. Freezing the specimen
Explanation
Answer: B - The point at which sections may then be cut at 10 microns of thickness
In the cold knife procedure, the term called as “Dew Line” is the point at which sections may then be cut at 10 microns of thickness. In this procedure, the tissue is lifted up to the knife manually and trimmed until the surface is flat. The exterior side is then brought to a warmer temperature by the use of one’s fingers until the tissue that was frozen will be eventually thawed by such action and as a result, it will be clearly visible. All the other options do not accurately describe the “Dew Line”.
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