HT Histotechnician

Answer: B - Immerse the old slide in Xylene for 24 hours, remove the cover slip, immerse in Xylene for another 30 minutes, immerse in potassium permanganate for 5-10 minutes, rinse with water for 5 minutes, immerse in 5% oxalic acid for 5 minutes, rinse, and re-stain with the appropriate stain.

A histotechnician needs to re-stain an old slide for repeat microscopic analysis of a Pathologist. The proper order before re-staining and the proper reagents to be used are indicated in the following: Immerse the old slide in Xylene for 24 hours, remove the cover slip, immerse in Xylene for another 30 minutes, immerse in potassium permanganate for 5-10 minutes, rinse with water for 5 minutes, immerse in 5% oxalic acid for 5 minutes, rinse, and re-stain with the appropriate stain. The other choices are incorrect and will cause the disintegration or the incomplete removal of the old stain from the old slide.