The rate-limiting step in glycolysis occurs at the step catalyzed by which of the following enzymes?
  1. glyceraldehyde-3-phosphate dehydrogenase
  2. 6-phosphofructo-1 kinase, PFK-1
  3. 6-PFK-2
  4. phosphoglycerate kinase
  5. pyruvate kinase
Explanation
Answer: B - The primary site of regulation of glycolysis occurs at the level of the PFK-1 catalyzed step. Hence, this reaction is the rate-limiting step in glycolysis.
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