IB Biology Exam Prep

Category - Biology

Which of the following techniques is used to increase the quantity of DNA in a sample to be analyzed?
  1. DNA sequencing.
  2. Polymerase chain reaction.
  3. Restriction fragment analysis.
  4. DNA hybridization.
Explanation
Correct Response: B. To increase the quantity of DNA in a sample, a scientist would use the polymerase chain reaction technique, in which the polymerase enzyme replicates samples of DNA. DNA sequencing (A) provides information about the genome but does not increase the quantity of DNA in a sample. Restriction fragment analysis (C), which provides information about DNA that has been cut into smaller fragments, is not a technique that increases the quantity of DNA in a sample. Likewise, the quantity of a DNA sample is not increased using the technique of DNA hybridization (D), which is a process that combines two single strands of DNA molecules into one single double-strand molecule.
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