When using steam sterilization, which of the following is necessary to properly remove microorganisms through the steam autoclave process?
  1. Steam, high temperature, and ethylene oxide gas
  2. Steam, high temperature, and pressure
  3. Steam, low temperature, and hydrogen peroxide
  4. Steam only
Explanation
Answer: B-Steam, high temperature, and pressure are necessary to properly remove microorganisms during the steam autoclave process. The pressure associated with steam incapacitates the microorganisms found on the items being autoclaved. This method of sterilization is commonly used in healthcare centers because of ease of use and effectiveness in killing pathogens.
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