Veterinary Technician

Category - Lab Procedures

Which of the following statements is true about a Ziehl - Neelsen Acid Fast Stain?
  1. It is made using Giemsa powder.
  2. Crystal Violet is the primary stain.
  3. Iodine is poured onto the slide and allowed to sit for 30 seconds.
  4. The slide is flooded with carbol-fuchsin stain and heated.
Explanation
Answer: D - Ziehl-Neelsen Acid-Fast Stain uses carbol-fuchsin stain, acid-alcohol decolorizer, and methylene blue counterstain. The first step in slide preparation is to flood the slide with carbol-fuchsin stain and heat.
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