Veterinary Technician

Category - Lab Procedures

When measuring plasma protein concentrations using a handheld refractometer, an erroneously high protein measurement will result from which of the following?
  1. Holding the refractometer up to a bright light source
  2. Using a lipemic or hemolyzed plasma sample
  3. Applying too much distilled water when cleaning the refractometer
  4. Failure to store the refractometer properly between uses
Explanation
Answer: B - Using a lipemic or hemolyzed plasma sample will cause an erroneously-high protein measurement. Both of these conditions cause the refractive index to be increased. Refractometer results are usually very accurate, providing that the plasma sample is clear.
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