Question 1

molecules of DNA from two different species that are inserted into a host organism to produce new genetic combinations that are of value to science, medicine, agriculture, and industry. ​

Accepted Answer

Recombinant DNA

Question 2

Recombinant DNA Technology is also called?

Accepted Answer

Genetic Engineering​

Question 3

tools of Recombinant DNA Technology​

Accepted Answer

1. Restriction  Enzymes 
2. Vector 
3. Host

Question 4

play a major role in determining the location at which the desired gene is inserted into the vector genome.​

Accepted Answer

Restriction  Enzymes

Question 5

Under the Restriction Enzyme there are

Accepted Answer

Endonuclease and Exonuclease

Question 6

cut within the DNA strand; they scrutinize the length of DNA and make the cut at the specific site—the restriction site. This gives rise to sticky ends in the sequence.​

Accepted Answer

Endonuclease

Question 7

remove the nucleotides from the ends of the strands.​

Accepted Answer

Exonuclease

Question 8

helps to synthesize.

Accepted Answer

Polymerase

Question 9

helps to bind.

Accepted Answer

Ligase

Question 10

carrier | help in carrying and integrating the desired gene; “ultimate vehicles” that carry forward the desired gene into the host organism. (plasmids, viruses, yeast cells)

Accepted Answer

​Vector

Question 11

into which the recombinant DNA is introduced; takes in the vector engineered with the desired DNA with the help of the enzymes. (bacterial cells)​

Accepted Answer

Host

Question 12

Process of Recombinant DNA Technology

Accepted Answer

1. Isolation of Genetic Material​.
2. Cutting the Gene at the recognition site​. 
3. Amplifying the gene copies through Polymerase chain reaction (PCR).​ 
4. Ligation of DNA Molecules.​ 
5. Insertion of Recombinant DNA into the host

Question 13

Application of rDNA

Accepted Answer

1. Health Sector 
2. Agriculture 
3. Food Industry

Question 14

treating growth disorders in children.

Accepted Answer

Human Growth Hormone (hGH)

Question 15

helps manage anemia in patients with kidney failure and cancer.

Accepted Answer

​Erythropoietin (EPO)

Question 16

is used to dissolve blood clots in stroke patients.​

Accepted Answer

Tissue Plasminogen Activator (tPA)

Question 17

This rDNA-made antibody targets HER2-positive breast cancer cells.

Accepted Answer

Herceptin (Trastuzumab)

Question 18

treats patients with hemophilia A, promoting blood clotting.

Accepted Answer

Factor VIII

Question 19

express a bacterial gene from Bacillus thuringiensis, which produces a protein toxic to certain insect pests.  (offer built-in insect resistance)​

Accepted Answer

Bt Crops

Question 20

variety of rice that produces beta-carotene, a precursor of Vitamin A. This biofortified rice aims to combat Vitamin A deficiency in populations relying heavily on rice as a staple food.

Accepted Answer

Golden Rice

Question 21

like soybeans and corn, are designed to be resistant to the herbicide glyphosate. This allows farmers to control weeds more effectively, as the crops can withstand glyphosate treatment, reducing the environmental impact of herbicides.​

Accepted Answer

​ Roundup Ready Crops

Question 22

Chymosin (Rennet)

Accepted Answer

Chymosin (Rennet)

Question 23

rDNA technology has been applied to enhance the enzymatic conversion of corn starch into glucose and fructose. This process leads to the production of ?, a sweetener used in a wide range of food products.​

Accepted Answer

High-Fructose Corn Syrup (HFCS) Production

Question 24

Recombinant DNA technology is employed to produce various ? and enzymes. For instance, certain enzymes used in food processing, such as amylases and proteases, can be manufactured using rDNA methods, ensuring consistent and safe production.

Accepted Answer

Bioengineered Food Additives